The E295K Cancer Variant of Human Polymerase Favors the

The E295K Cancer Variant of Human Polymerase Favors the

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Unfavorable Electrostatic and Steric Interactions in DNA

The E295K Cancer Variant of Human Polymerase Favors the Mismatch Conformational Pathway during Nucleotide Selection.Journal of Biological Chemistry 2013,288 (48) ,34850-34860.DOI 10.1074/jbc.M113.510891.Tamar Schlick,Karunesh Arora,William A.Beard,Samuel H.Wilson.Unfavorable Electrostatic and Steric Interactions in DNA The E295K Cancer Variant of Human Polymerase Favors the Mismatch Conformational Pathway during Nucleotide Selection.Journal of Biological Chemistry 2013 ,288 (48) ,34850-34860.Unfavorable Electrostatic and Steric Interactions in DNA E295K appears to lack DNA polymerase activity as a result of its inability to assume an active polymerase conformation [28,29].During BER,neither of these variants fills single nucleotide gaps

The E295K DNA polymerase beta gastric cancer-associated

The E295K DNA polymerase beta gastric cancer-associated variant interferes with base excision repair and induces cellular transformation.Lang T(1),Dalal S,Chikova A,DiMaio D,Sweasy JB.Author information (1)Department of Therapeutic Radiology,Yale University School of Medicine,15 York Street,P.O.Box 20840,New Haven,CT 06520,USA.The E295K DNA polymerase beta gastric cancer-associated Approximately 30% of human tumors examined for mutations in polymerase beta (pol beta) appear to express pol beta variant proteins (D.Starcevic,S.Dalal,and J.B.Sweasy,Cell Cycle 3:998-1001,2004).Many of these variants result from a single amino acid substitution.The E295K Cancer Variant of Human Polymerase beta Favors the Mismatch Conformational Pathway during Nucleotide Selection.DNA polymerase (pol ) is responsible for gap filling synthesis during repair of damaged DNA as part of the base excision repair pathway.Author:Brian E.Eckenroth,Jamie B.Towle-Weicksel,Joann B.Sweasy,Sylvie Doubli The E295K Cancer Variant of Human Polymerase Favors the#233;Cited by:13Publish Year:2013RCSB PDB - 4M9N DNA Polymerase Beta E295K Soaked witWas this helpful?The E295K Cancer Variant of Human Polymerase Favors the Nov 29,2013 The E295K Cancer Variant of Human Polymerase Favors the#0183;The E295K Cancer Variant of Human Polymerase Favors the Mismatch Conformational Pathway during Nucleotide Selection * Brian E.Eckenroth, Jamie B.Towle-Weicksel, The E295K Cancer Variant of Human Polymerase Favors the#167; Joann B.Sweasy, The E295K Cancer Variant of Human Polymerase Favors the#167;,1 and Sylvie Doubli The E295K Cancer Variant of Human Polymerase Favors the#233; ,2 From the Department of Microbiology and Molecular Genetics,University of Vermont,Burlington,Vermont 05405 and

Structure-function analysis of the mammalian DNA

The E295K Cancer Variant of Human Polymerase Favors the Mismatch Conformational Pathway during Nucleotide Selection.Journal of Biological Chemistry 2013 ,288 (48) ,34850-34860.Structure and Mechanism of DNA Polymerase ChemicalThe E295K Cancer Variant of Human Polymerase ? Favors the Mismatch Conformational Pathway during Nucleotide Selection.Journal of Biological Chemistry 2013,288 (48) ,34850-34860.DOI 10.1074/jbc.M113.510891.Pooria Farahani,Marcus Lundberg,Hans O.Karlsson.Structure and Mechanism of DNA Polymerase ChemicalThe E295K Cancer Variant of Human Polymerase ? Favors the Mismatch Conformational Pathway during Nucleotide Selection.Journal of Biological Chemistry 2013 ,288 (48) ,34850-34860.

RCSB PDB - 4M9N DNA Polymerase Beta E295K Soaked with

The E295K Cancer Variant of Human Polymerase beta Favors the Mismatch Conformational Pathway during Nucleotide Selection.DNA polymerase (pol ) is responsible for gap filling synthesis during repair of damaged DNA as part of the base excision repair pathway.RCSB PDB - 4M9L DNA Polymerase Beta E295K SoakedDownload Endnote The E295K Cancer Variant of Human Polymerase beta Favors the Mismatch Conformational Pathway during Nucleotide Selection.Eckenroth,B.E.,Towle-Weicksel,J.B.,Sweasy,J.B.,Doublie,S.(2013) J Biol Chem 288 34850-34860Previous123456NextThe E295K DNA polymerase beta gastric cancer-associated Approximately 30% of human tumors examined for mutations in polymerase beta (pol beta) appear to express pol beta variant proteins (D.Starcevic,S.Dalal,and J.B.Sweasy,Cell Cycle 3:998-1001,2004).Many of these variants result from a single amino acid substitution.

Pre-Steady-State Kinetic Studies of the Fidelity and

Apr 30,2004 The E295K Cancer Variant of Human Polymerase Favors the#0183;The E295K Cancer Variant of Human Polymerase Favors the Mismatch Conformational Pathway during Nucleotide Selection.Journal of Biological Chemistry 2013 ,288 (48) ,Pre-Steady-State Kinetic Studies of the Fidelity and Apr 30,2004 The E295K Cancer Variant of Human Polymerase Favors the#0183;The E295K Cancer Variant of Human Polymerase Favors the Mismatch Conformational Pathway during Nucleotide Selection.Journal of Biological Chemistry 2013 ,288 (48) ,Polbase - Polbase Reference BrowserThe E295K cancer variant of human polymerase beta favors the mismatch conformational pathway during nucleotide selection.Brian E Eckenroth,Jamie B Towle-Weicksel,Joann B Sweasy,Sylvie Doubli Eacute The Journal of biological chemistry 2013 topics set

Polbase - Authors

The E295K cancer variant of human polymerase beta favors the mismatch conformational pathway during nucleotide selection.Sweasy JB 2013 The Journal of biological chemistry Structural changes in the hydrophobic hinge region adversely affect the activity and fidelity of the I260Q mutator of DNA polymerase The E295K Cancer Variant of Human Polymerase Favors the#223;New structural snapshots provide molecular insights into Aug 01,2015 The E295K Cancer Variant of Human Polymerase Favors the#0183;The closed/open equilibrium is also altered by another mutation,the E295K variant. S.Doubli The E295K Cancer Variant of Human Polymerase Favors the#233;The E295K cancer variant of human polymerase favors the mismatch conformational pathway during nucleotide selection.J.Biol.Chem.,288 (2013),pp.34850-34860.Google Scholar.New Structural Snapshots Provide Molecular Insights into Eckenroth BE,Towle-Weicksel JB,Sweasy JB,Doubli The E295K Cancer Variant of Human Polymerase Favors the#233; S.The E295K cancer variant of human polymerase favors the mismatch conformational pathway during nucleotide selection.J Biol Chem.2013; 288:3485034860.[PMC free article]

New Structural Snapshots Provide Molecular Insights into

Eckenroth BE,Towle-Weicksel JB,Sweasy JB,Doubli The E295K Cancer Variant of Human Polymerase Favors the#233; S.The E295K cancer variant of human polymerase favors the mismatch conformational pathway during nucleotide selection.J Biol Chem.2013; 288:3485034860.[PMC free article]Joann B.Sweasy - PublicationsThe E295K cancer variant of human polymerase favors the mismatch conformational pathway during nucleotide selection.The Journal of Biological Chemistry.288 34850-60.PMID 24133209 DOI 10.1074/jbc.M113.510891 1 2013 Gridley CL,Rangarajan S,Firbank S,Dalal S,Sweasy JB,Jaeger J.Structural changes in the hydrophobic hinge region Jamie B.Towle-Weicksel - PublicationsThe E295K cancer variant of human polymerase favors the mismatch conformational pathway during nucleotide selection.The Journal of Biological Chemistry.288 34850-60.PMID 24133209 DOI 10.1074/jbc.M113.510891 0.44 Show low-probability matches.

Human POLB Gene Is Mutated in High Percentage of

Jul 06,2012 The E295K Cancer Variant of Human Polymerase Favors the#0183;One tumor also has the E295K variant,which has been previously identified in a gastric tumor and has been shown to be an inactive polymerase that induces genomic instability and cellular transformation (25,33).Interestingly,82% of the missense mutations identified in this study are CG to TA transitions and are suggestive of mutagenesis DNA Polymerase Beta Cancer-Associated Variant I260M Eckenroth BE,Towle-Weicksel JB,Sweasy JB,Doublie S.The E295K cancer variant of human polymerase beta favors the mismatch conformational pathway during nucleotide selection.J Biol Chem.2013; 288:3485034860.[PMC free article]Crystal structure of DNA polymerase with DNA containing The E295K Cancer Variant of Human Polymerase Favors the#0183;The closed/open equilibrium is also altered by another mutation,the E295K variant. S.Doubli The E295K Cancer Variant of Human Polymerase Favors the#233;The E295K cancer variant of human polymerase favors the mismatch conformational pathway during nucleotide selection.J.Biol.Chem.,288 (2013),pp.34850-34860.Google Scholar.

Crystal Structure of DNA Polymerase with DNA Containing

The resulting poor binding could be due to the lesion itself or the nucleotide binding properties of E295K.14 An additional mutation akin to the Y567A variant in RB69 gp43,which opened the polymerase active site and was necessary to visualize a GhdATP ternary complex,10 may be necessary to capture a ternary complex with pol bound to (S Crystal Structure of DNA Polymerase with DNA Containing The resulting poor binding could be due to the lesion itself or the nucleotide binding properties of E295K.14 An additional mutation akin to the Y567A variant in RB69 gp43,which opened the polymerase active site and was necessary to visualize a GhdATP ternary complex,10 may be necessary to capture a ternary complex with pol bound to (S Cited by 379Publish Year 2006Author William A.Beard,Samuel H.Wilson4m9h.1 SWISS-MODEL Template LibrarySWISS-MODEL Template Library (SMTL) entry for 4m9h.1.DNA Polymerase Beta E295K Soaked with dTTP

Cited by 13Publish Year 2013Author Brian E.Eckenroth,Jamie B.Towle-Weicksel,Joann B.Sweasy,Sylvie Doubli The E295K Cancer Variant of Human Polymerase Favors the#233;The E295K DNA polymerase beta gastric cancer-associated

In the work described here,we show that the E295K gastric carcinoma pol beta variant acts in a dominant-negative manner by interfering with base excision repair.This leads to an increase in sister chromatid exchanges.Expression of the E295K variant also induces cellular transformation.Cited by 13Publish Year 2013Author Brian E.Eckenroth,Jamie B.Towle-Weicksel,Joann B.Sweasy,Sylvie Doubli The E295K Cancer Variant of Human Polymerase Favors the#233;The E295K Cancer Variant of Human Polymerase Favors the The E295K Cancer Variant of Human Polymerase Favors the Mismatch Conformational Pathway during Nucleotide Selection Article in Journal of Biological Chemistry 288(48) The E295K Cancer Variant of Human Polymerase Favors the#160; The E295K Cancer Variant of Human Polymerase Favors the#160;October 2013 with 21Cancer-Associated DNA Polymerase Beta VariantsE295K result in cellular transformation. Polymerase Beta Variants During Base Excision Repair ROS,ALKYLATING AGENTS C C G C.Mutator Phenotype Hypothesis Loeb LA et al.(1974) Cancer Res.34:2311-2321 of human cancer.Daniel DiMaio CA16038.Title Microsoft PowerPoint - sweasynih.ppt

A Structural Solution for the DNA Polymerase -Dependent

The E295K Cancer Variant of Human Polymerase Favors the Mismatch Conformational Pathway during Nucleotide Selection. We showed that expression of the carcinoma variant E295K12345NextRCSB PDB - 4M9J DNA Polymerase Beta E295K SoakedThe E295K Cancer Variant of Human Polymerase beta Favors the Mismatch Conformational Pathway during Nucleotide Selection. Characterization of specific cancer variants is particularly useful to further the understanding of the general mechanism of pol while providing context to disease contribution.We showed that expression of the (PDF) A Change in the Rate-Determining Step of The E295K cancer variant of human .polymerase beta favors the mismatch conformational pathway during nucleotide selection.J Biol .Chem.2013; 288:3485034860.[PubMed 24133209]

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